Exosome Isolation

Lihan Biologics, with its expertise in exosome research, provides customized exosome isolation services to concentrate and purify exosome samples. We isolate exosomes from a wide spectrum of cellular debris and almost all types of biofluids. Our results are reliable and reproducible owing to our well-established technologies and experienced team of scientists. Isolation is done from the following sources but is not limited to:

Culture media
Plasma
Urine
Serum
CSF
Saliva
Plants
Follicular Fluid
Marine Organisms

We use wide range of methods for exosome isolation depending upon the requirement in terms of yield and specificity.

Culture media
Precipitation: High yield, Maintain vesicle morphology
Ultrafiltration (Diafiltration and Tangential Flow Filtration (TFF): Pure exosome preparations, No limit of sample volume, Reduced filter clogging
Size-exclusion Chromatography: Reproducible, Preserves vesicle integrity, High sample recovery, Extremely customizable (size of pore, volume of column, length of column)
Ultracentrifugation: Isolation from large volumes, No added chemicals
Density Gradient Centrifugation (Sucrose or Idoixanol): Very pure populations (Iodixanol can separate exosomes from virions), Intact vesicle morphology
Immunoaffinity capture: High Purity, High Selectivity