Exosome Purification

Exosomes can be found in most body fluids, including urine, saliva, amniotic fluid, semen, breast milk, plasma, and blood, making them a promising basis for the minimally invasive detection through biofluids. We have validated various purification methods which can specifically capture exosomes and isolate high purity exosomes from cell culture medium and body fluids. The purified intact exosomes can be characterized by electron microscopic analysis, nanoparticle tracking analysis, and other protein and molecular analyses such as Western blotting and sequencing. To enhance the concentration and quality of exosomes, many techniques have been developed for exosome purification, including ultracentrifugation and microfiltration, size exclusion chromatography (SEC), and immunoaffinity capture.